Falanqaynta raadraaca muunadaha dareeraha ah waxay leedahay codsiyo badan oo kala duwan oo ku saabsan cilmiga nolosha iyo la socodka deegaanka.Shaqadan, waxaanu ku samaynay sawir-qaade is haysta oo jaban oo ku salaysan birta waveguide capillaries (MCCs) ee go'aaminta xasaasiga ah ee nuugista.Jidka indhaha ayaa si weyn loo kordhin karaa, oo aad uga dheer dhererka jirka ee MWC, sababtoo ah iftiinka kala firirsan darbiyada birta ah ee siman ayaa ku jiri kara gudaha xididka iyada oo aan loo eegin xagasha dhacdada.Xoojinta sida ugu hooseysa 5.12 nM waxaa lagu gaari karaa iyadoo la isticmaalayo reagents chromogenic caadiga ah sababtoo ah kordhinta indhaha cusub ee aan toosan ahayn iyo muunada degdega ah ee beddelka iyo ogaanshaha gulukooska.
Photometry waxaa si weyn loogu isticmaalaa baaritaanka raadraaca ee muunado dareere ah sababta oo ah tirada badan ee reagents chromogenic la heli karo iyo qalab optoelectronic semiconductor1,2,3,4,5.Marka la barbar dhigo go'aaminta nuugista ku salaysan cuvette-dhaqameedka, dareeraha waveguide (LWC) koofiyadaha ayaa ka tarjumaya (TIR) iyaga oo ilaalinaya iftiinka baaritaanka gudaha xididka1,2,3,4,5.Si kastaba ha ahaatee, iyada oo aan horumarin dheeraad ah, dariiqa indhaha ayaa kaliya ku dhow dhererka jirka ee LWC3.6, iyo kordhinta dhererka LWC ee ka baxsan 1.0 m waxay la kulmi doontaa iftiin xoog leh iyo khatarta sare ee xumbo, iwm 3, 7. Iyadoo la tixgelinayo. unugga milicsiga badan ee la soo jeediyay ee hagaajinta dariiqa indhaha, xadka ogaanshaha waxaa lagu hagaajiyay kaliya 2.5-8.9.
Hadda waxaa jira laba nooc oo waaweyn oo LWC ah, kuwaas oo kala ah Teflon AF capillaries (oo leh index refractive kaliya ~ 1.3, taas oo ka hooseysa kan biyaha) iyo silica capillaries oo lagu dahaadhay Teflon AF ama filimada birta ah1,3,4.Si loo gaaro TIR ee is dhexgalka u dhexeeya walxaha dielectric, qalabka leh index refractive hoose iyo xaglaha dhacdooyinka iftiinka sare ayaa loo baahan yahay3,6,10.Marka la eego kaabadaha Teflon AF, Teflon AF waa neefsasho sababtoo ah qaabdhismeedkiisa dalool3,11 wuxuuna nuugi karaa xaddi yar oo maaddooyin ah muunadaha biyaha.Xididdada quartz ee dibadda ku dahaadhay Teflon AF ama birta, index refractive ee quartz (1.45) ayaa ka sarreeya inta badan muunadaha dareeraha ah (tusaale 1.33 biyaha)3,6,12,13.Xididdada lagu dahaadhay filim bir ah oo gudaha ah, guryaha gaadiidka ayaa la darsay14,15,16,17,18, laakiin habka dahaadhku waa adag yahay, dusha sare ee filimka birta ah wuxuu leeyahay qaab-dhismeed qallafsan oo jilicsan4,19.
Intaa waxaa dheer, LWC-yada ganacsiga (AF Teflon Coated Capillaries iyo AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) waxay leeyihiin faa'iidooyin kale, sida: khaladaadka..Mugga weyn ee dhintay TIR3,10, (2) xiriiriyaha T (si loogu xidho xididada, fiilooyinka, iyo tuubooyinka meesha laga soo galo) ayaa dabin kara goobooyin hawo10.
Isla mar ahaantaana, go'aaminta heerarka gulukooska ayaa muhiimad weyn u leh ogaanshaha cudurka macaanka, cirrhosis ee beerka iyo cudurrada dhimirka20.iyo habab badan oo lagu ogaanayo sida sawir-qaadista (oo ay ku jiraan spectrophotometry 21, 22, 23, 24, 25 iyo colorimetry warqad 26, 27, 28), galvanometry 29, 30, 31, fluorometry 32, 33, 34, 35, polarimetry 3. resonance balasman.37, Fabry-Perot godka 38, electrochemistry 39 iyo electrophoresis capillary 40,41 iyo wixii la mid ah.Si kastaba ha ahaatee, hababkan intooda badan waxay u baahan yihiin qalab qaali ah, iyo ogaanshaha gulukooska ee dhowr nanomolar ayaa weli ah caqabad (tusaale ahaan, cabbirada photometric21, 22, 23, 24, 25, 26, 27, 28, fiirsashada ugu yar ee gulukooska).xaddidaadda waxay ahayd kaliya 30 nM markii nanoparticles buluug Prussian loo isticmaalay sidii peroxidase mimics).Falanqaynta gulukooska Nanomolar ayaa inta badan looga baahan yahay daraasadaha gacanta ee heerka-molecular sida ka hortagga kansarka qanjirka 'prostate' ee korriinka42 iyo habdhaqanka hagaajinta CO2 ee Prochlorococcus ee badda.
Waqtiga boostada: Nov-26-2022